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Diagn Microbiol Infect Dis. 2015 Feb;81(2):105-6. doi: 10.1016/j.diagmicrobio.2014.10.001. Epub 2014 Oct 7.

Improved serotype-specific dengue virus detection in Trinidad and Tobago using a multiplex, real-time RT-PCR.

Author information

1
Department of Medicine, Division of Infectious Diseases, Stanford University School of Medicine, Stanford, CA, USA.
2
Department of Pre-Clinical Studies, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago.
3
Trinidad Public Health Laboratory, Port of Spain, Trinidad and Tobago.
4
Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA.
5
Department of Medicine, Division of Infectious Diseases, Stanford University School of Medicine, Stanford, CA, USA; Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA. Electronic address: bpinsky@stanford.edu.

Abstract

Dengue virus (DENV) transmission occurs throughout the Caribbean, though laboratory confirmation and epidemiologic surveillance are limited by the availability of serotype-specific molecular diagnostics. In this study, we show that a serotype-specific DENV multiplex, real-time reverse transcriptase-PCR (RT-PCR) detected DENV RNA in significantly more samples (82/182) than a reference hemi-nested RT-PCR (57/182; P=0.01).

KEYWORDS:

Dengue; Molecular; Pathology

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