Send to

Choose Destination
Environ Toxicol. 2016 Jul;31(7):877-86. doi: 10.1002/tox.22099. Epub 2014 Dec 23.

MicroRNA-155 targets cyb561d2 in zebrafish in response to fipronil exposure.

Author information

College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, People's Republic of China.
Department of Applied Engineering, Zhejiang Economic & Trade Polytechnic, Hangzhou, 310018, People's Republic of China.
Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou, 310029, People's Republic of China.


MicroRNAs (miRNAs), which are a class of small noncoding RNAs, can modulate the expression of many protein-coding genes when an organism is exposed to an environmental chemical. We previously demonstrated that miR-155 was significantly downregulated in adult zebrafish (Danio rerio) in response to fipronil (5-amino-1-[2,6-dichloro-4-(trifluoromethyl) phenyl]-4-[(trifluoromethyl) sulphinyl]-1H-pyrazole-3-carbonitrile) exposure. However, the regulation of this miRNA's predicted target gene cyb561d2, which is a member of the cytochrome b561 (cyt b561) family involved in electron transfer, cell defence, and chemical stress, has not been experimentally validated to date. In this study, we evaluated the effects of fipronil on miR-155 and cyb561d2 in zebrafish. The expression of miR-155 was downregulated, whereas cyb561d2 was upregulated in both mRNA and protein level in a dose-dependent manner upon stimulation of fipronil. The dual luciferase report assay demonstrated that miR-155 interacted with cyb561d2 3'-untranslated regions (3'-UTR). The expression of cyb561d2 was reduced in both mRNA and protein levels when ZF4 cells were transfected with an miR-155 mimic, whereas its expression levels of both mRNA and protein were increased when endogenous miR-155 was inhibited by transfection with an miR-155 inhibitor. The results improved our understanding of molecular mechanism of toxicity upon fipronil exposure, and presents miR-155 as a potential novel toxicological biomarker for chemical exposure.


cyb561d2; fipronil; mechanism of toxicity; miR-155; zebrafish

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center