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Org Lett. 2015 Jan 16;17(2):187-9. doi: 10.1021/ol503320p. Epub 2014 Dec 22.

A second-generation phosphohistidine analog for production of phosphohistidine antibodies.

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  • 1Department of Chemistry, Princeton University , Princeton, New Jersey 08544, United States.

Abstract

Protein histidine phosphorylation plays a crucial role in cell signaling and central metabolism. However, its detailed functions remain elusive due to technical challenges in detecting and isolating proteins bearing phosphohistidine (pHis), a labile posttranslational modification (PTM). To address this issue, we previously developed the first pHis-specific antibodies using stable, synthetic triazole-based pHis analogs. A second-generation, pyrazole-based pHis analog that enabled the development of a pan-pHis antibody with much improved pHis specificity is now reported.

PMID:
25531910
DOI:
10.1021/ol503320p
[PubMed - indexed for MEDLINE]
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