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Bioanalysis. 2014;6(23):3081-9. doi: 10.4155/bio.14.254.

Determination of the antimalarial drug piperaquine in small volume pediatric plasma samples by LC-MS/MS.

Author information

1
Drug Research Unit, Department of Clinical Pharmacy, University of California at San Francisco, San Francisco, CA 94143, USA.

Abstract

AIM:

Determination of piperaquine (PQ) in pediatric plasma requires a method with a small sample volume.

RESULTS:

We report a sensitive LC-MS/MS method for quantitation of PQ with only 25 µl human plasma. Using a deuterated internal standard (PQ-d6), an analytical PFP column, APCI(+) as the ion source and MRM (535/288 for PQ and 541/294 for the IS) for detection, the method has a linear calibration range of 1.5-250 ng/ml with a runtime of 3.0 min per sample. The method was applied to plasma samples from children.

CONCLUSION:

The developed LC-MS/MS method is suitable for pediatric studies with small volume plasma samples collected via capillary tubes. One limitation was the performance of PFP columns varied among different brands.

PMID:
25529877
PMCID:
PMC4321809
DOI:
10.4155/bio.14.254
[Indexed for MEDLINE]
Free PMC Article

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