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Virology. 2015 Feb;476:54-60. doi: 10.1016/j.virol.2014.11.019. Epub 2014 Dec 17.

Repertoire of virus-derived small RNAs produced by mosquito and mammalian cells in response to dengue virus infection.

Author information

1
Department of Biology, New Mexico State University, Las Cruces, NM, USA. Electronic address: eesem@vet.k-state.edu.
2
Department of Biology, New Mexico State University, Las Cruces, NM, USA. Electronic address: andradec@willamette.edu.
3
National Center for Genome Resources (NCGR), Santa Fe, NM, USA. Electronic address: npd@ncgr.org.
4
National Center for Genome Resources (NCGR), Santa Fe, NM, USA. Electronic address: tr@ncgr.org.
5
National Center for Genome Resources (NCGR), Santa Fe, NM, USA. Electronic address: jlj@ncgr.org.
6
National Center for Genome Resources (NCGR), Santa Fe, NM, USA. Electronic address: fds@ncgr.org.
7
Department of Biology, New Mexico State University, Las Cruces, NM, USA. Electronic address: khanley@nmsu.edu.

Abstract

RNA interference (RNAi) is the major defense of many arthropods against arthropod-borne RNA viruses (arboviruses), but the role of RNAi in vertebrate immunity to arboviruses is not clear. RNA viruses can trigger RNAi in vertebrate cells, but the vertebrate interferon response may obscure this interaction. We quantified virus-derived small RNAs (vRNAs) generated by mosquito (U4.4) cells and interferon-deficient (Vero) and interferon-competent (HuH-7) mammalian cells infected with a single isolate of mosquito-borne dengue virus. Mosquito cells produced significantly more vRNAs than mammalian cells, and mosquito cell vRNAs were derived from both the positive- and negative-sense dengue genomes whereas mammalian cell vRNAs were derived primarily from positive-sense genome. Mosquito cell vRNAs were predominantly 21 nucleotides in length whereas mammalian cell vRNAs were between 12 and 36 nucleotides with a modest peak at 24 nucleotides. Hot-spots, regions of the virus genome that generated a disproportionate number of vRNAs, overlapped among the cell lines.

KEYWORDS:

Dengue; Flavivirus; HuH-7; Next-generation sequencing; RNA interference; U4.4; Vero; Virus; siRNA; viRNA

PMID:
25528416
PMCID:
PMC4323773
DOI:
10.1016/j.virol.2014.11.019
[Indexed for MEDLINE]
Free PMC Article

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