Send to

Choose Destination
Am J Clin Nutr. 2015 Jan;101(1):44-54. doi: 10.3945/ajcn.114.094219. Epub 2014 Nov 19.

Impact of a 6-wk olive oil supplementation in healthy adults on urinary proteomic biomarkers of coronary artery disease, chronic kidney disease, and diabetes (types 1 and 2): a randomized, parallel, controlled, double-blind study.

Author information

From the Analytical Services Unit, Instituto de Biologia Experimental Tecnologica, Oeiras, Portugal (SS and MRB); the Analytical Chemistry Department, Instituto de Tecnologia Química e Biológica, Oeiras, Portugal (SS and MRB); the Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal (SS and MRB); the Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal (MRB and MEF); Mosaiques Diagnostics AG, Hannover, Germany (JS and HM); and Human Nutrition, School of Medicine (EC) and the Institute of Cardiovascular and Medical Sciences (WM), College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom.



Olive oil (OO) consumption is associated with cardiovascular disease prevention because of both its oleic acid and phenolic contents. The capacity of OO phenolics to protect against low-density lipoprotein (LDL) oxidation is the basis for a health claim by the European Food Safety Authority. Proteomic biomarkers enable an early, presymptomatic diagnosis of disease, which makes them important and effective, but understudied, tools for primary prevention.


We evaluated the impact of supplementation with OO, either low or high in phenolics, on urinary proteomic biomarkers of coronary artery disease (CAD), chronic kidney disease (CKD), and diabetes.


Self-reported healthy participants (n = 69) were randomly allocated (stratified block random assignment) according to age and body mass index to supplementation with a daily 20-mL dose of OO either low or high in phenolics (18 compared with 286 mg caffeic acid equivalents per kg, respectively) for 6 wk. Urinary proteomic biomarkers were measured at baseline and 3 and 6 wk alongside blood lipids, the antioxidant capacity, and glycation markers.


The consumption of both OOs improved the proteomic CAD score at endpoint compared with baseline (mean improvement: -0.3 for low-phenolic OO and -0.2 for high-phenolic OO; P < 0.01) but not CKD or diabetes proteomic biomarkers. However, there was no difference between groups for changes in proteomic biomarkers or any secondary outcomes including plasma triacylglycerols, oxidized LDL, and LDL cholesterol.


In comparison with low-phenolic OO, supplementation for 6 wk with high-phenolic OO does not lead to an improvement in cardiovascular health markers in a healthy cohort.


Mediterranean diet; coronary artery disease; olive oil; phenolics; proteomic biomarkers

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Silverchair Information Systems
Loading ...
Support Center