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Cell. 2014 Dec 18;159(7):1652-64. doi: 10.1016/j.cell.2014.11.045.

Detecting envelope stress by monitoring β-barrel assembly.

Author information

1
WELBIO, Université catholique de Louvain, Avenue Hippocrate 75, Brussels 1200, Belgium; de Duve Institute, Université catholique de Louvain, Avenue Hippocrate 75, Brussels 1200, Belgium.
2
European Molecular Biology Laboratory, Genome Biology Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
3
de Duve Institute, Université catholique de Louvain, Avenue Hippocrate 75, Brussels 1200, Belgium.
4
European Molecular Biology Laboratory, Genome Biology Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany. Electronic address: typas@embl.de.
5
WELBIO, Université catholique de Louvain, Avenue Hippocrate 75, Brussels 1200, Belgium; de Duve Institute, Université catholique de Louvain, Avenue Hippocrate 75, Brussels 1200, Belgium. Electronic address: jfcollet@uclouvain.be.

Abstract

The cell envelope protects bacteria from their surroundings. Defects in its integrity or assembly are sensed by signal transduction systems, allowing cells to rapidly adjust. The Rcs phosphorelay responds to outer membrane (OM)- and peptidoglycan-related stress in enterobacteria. We elucidated how the OM lipoprotein RcsF, the upstream Rcs component, senses envelope stress and activates the signaling cascade. RcsF interacts with BamA, the major component of the β-barrel assembly machinery. In growing cells, BamA continuously funnels RcsF through the β-barrel OmpA, displaying RcsF on the cell surface. This process spatially separates RcsF from the downstream Rcs component, which we show is the inner membrane protein IgaA. The Rcs system is activated when BamA fails to bind RcsF and funnel it to OmpA. Newly synthesized RcsF then remains periplasmic, interacting with IgaA to activate the cascade. Thus RcsF senses envelope damage by monitoring the activity of the Bam machinery.

PMID:
25525882
DOI:
10.1016/j.cell.2014.11.045
[Indexed for MEDLINE]
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