Format

Send to

Choose Destination
G3 (Bethesda). 2014 Dec 18;5(2):253-9. doi: 10.1534/g3.114.015156.

Plant genetic archaeology: whole-genome sequencing reveals the pedigree of a classical trisomic line.

Author information

1
Department of Molecular Biology, Max Planck Institute for Developmental Biology, D-72076 Tübingen, Germany salome@chem.ucla.edu.
2
Department of Molecular Biology, Max Planck Institute for Developmental Biology, D-72076 Tübingen, Germany.

Abstract

The circadian oscillator is astonishingly robust to changes in the environment but also to genomic changes that alter the copy number of its components through genome duplication, gene duplication, and homeologous gene loss. While studying the potential effect of aneuploidy on the Arabidopsis thaliana circadian clock, we discovered that a line thought to be trisomic for chromosome 3 also bears the gi-1 mutation, resulting in a short period and late flowering. With the help of whole-genome sequencing, we uncovered the unexpected complexity of this trisomic stock's history, as its genome shows evidence of past outcrossing with another A. thaliana accession. Our study indicates that although historical aneuploidy lines exist and are available, it might be safer to generate new individuals and confirm their genomes and karyotypes by sequencing.

KEYWORDS:

GIGANTEA; circadian period; deep-sequencing; flowering time; trisomic line

PMID:
25524155
PMCID:
PMC4321033
DOI:
10.1534/g3.114.015156
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center