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J Med Assoc Thai. 2014 Feb;97 Suppl 2:S202-6.

Comparison of chromosome analysis using cell culture by coverslip technique with flask technique.

Abstract

OBJECTIVE:

To determine accuracy rate ofchromosome study from amniotic cellculture by coverslip technique compared with flask technique and to compared timing ofamniotic cell culture, amount ofamniotic cell culture media and cost ofamniotic cell culture.

STUDY DESIGN:

Cross sectional study.

SETTING:

Department of Obstetrics and Gynecology, Phramongkutklao Hospital. Subjects: 70 pregnant women who underwent amniocentesis at Phramongkutklao Hospital during November 1, 2007 to February 29, 2008.

INTERVENTION:

Amniotic cell culture by flask technique and coverslip technique.

MAIN OUTCOME MEASURES:

Accuracy of amniotic cell culture for chromosome study by coverslip technique compared with flask technique.

RESULTS:

Totally 70 pregnant women who underwent to amniocentesis and dividedamniotic fluid to cell culture by flask technique and coverslip technique. 69 samples had similar resultfrom both techniques. The only one sample had cell culture failure inboth methods due to blood contamination. Accuracy in coverslip technique was 100% compared with flask technique. In timing of amniotic cell culture, amount ofamniotic cell culture media and cost of amniotic cell culture between 2 methods that coverslip technique was lesser than flask technique.

CONCLUSION:

There is statistically significant of accuracy in chromosome result between coverslip technique and flask technique. Coverslip technique was lesser than flask technique in timing, amniotic cell culture media and costs ofamniotic cell culture.

PMID:
25518195
[Indexed for MEDLINE]

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