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Cell Stem Cell. 2014 Nov 6;15(5):546-58. doi: 10.1016/j.stem.2014.10.015. Epub 2014 Nov 6.

Quantitative single-cell approaches to stem cell research.

Author information

1
Department of Biosystems Science and Engineering, ETH Zurich, 4058 Basel, Switzerland.
2
Department of Biosystems Science and Engineering, ETH Zurich, 4058 Basel, Switzerland. Electronic address: timm.schroeder@bsse.ethz.ch.

Abstract

Understanding the molecular control of cell fates is central to stem cell research. Such insight requires quantification of molecular and cellular behavior at the single-cell level. Recent advances now permit high-throughput molecular readouts from single cells as well as continuous, noninvasive observation of cell behavior over time. Here, we review current state-of-the-art approaches used to query stem cell fate at the single-cell level, including advances in lineage tracing, time-lapse imaging, and molecular profiling. We also offer our perspective on the advantages and drawbacks of available approaches, key technical limitations, considerations for data interpretation, and future innovation.

PMID:
25517464
DOI:
10.1016/j.stem.2014.10.015
[Indexed for MEDLINE]
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