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Liver Int. 2015 Jul;35(7):1893-901. doi: 10.1111/liv.12763. Epub 2015 Jan 20.

Pretransplant urinary proteome analysis does not predict development of chronic kidney disease after liver transplantation.

Author information

1
Department of Nephrology and Organ Transplantation, CHU Rangueil, Toulouse, France.
2
U1048, Institut of Cardiovascular and Metabolic Disease, Institut National de la Santé et de la Recherche Médicale (INSERM), Toulouse, France.
3
Université Toulouse III Paul-Sabatier, Toulouse, France.
4
Institut of Cardiovascular and Metabolic Disease, Plateau de Protéomique des Liquides Biologiques, Toulouse, France.
5
HybridStat Predictive Analytics OE, Athens, Greece.
6
Mosaiques Diagnostics GmbH, Hannover, Germany.
7
Charite-Universitatsmedizin Berlin, Berlin, Germany.
8
Department of Surgery and Liver Transplantation, Toulouse, France.
9
Internal Medecine-Digestive Department, CHU Purpan, Toulouse, France.
10
UMR 152, IRD, Université Toulouse III Paul-Sabatier, Toulouse, France.
11
Department of Hepatology, Federation Digestive, CHU Purpan, Toulouse, France.
12
IFR-BMT, INSERM U1043, CHU Purpan, Toulouse, France.

Abstract

BACKGROUND & AIMS:

Chronic kidney disease (CKD) is a common complication after liver transplantation. Kidney biopsies cannot be easily performed before liver transplantation to predict patients at high risk for CKD. The aim of our study was to determine whether pre-, peri- and post-transplant factors, as well as peptides present in preliver transplant urine samples were associated with loss in kidney function at 6 months post-transplantation using proteome analysis.

METHODS:

Eighty patients who underwent a liver transplantation and that had pretransplant glomerular filtration rate (GFR) value of ≥60 mL/min/1.73 m² (MDRD) were included in the study.

RESULTS:

GFR decreased significantly after transplantation. At month 6 post-transplantation, 40 patients displayed a CKD, i.e. eGFR of <60 mL/min/1.73 m², while the other 40 patients did not. Although thousands of peptides were identified, none was significantly associated with the development of CKD at 6 months after liver transplantation. Moreover, using a urinary peptidome classifier to detect preexisting CKD, no difference was found in CKD scores between the 2 groups. After analysis of a large number of pre-, peri- and post-transplant parameters, viral hepatitis as a cause for liver transplantation was the sole independent predictive factor for CKD. No difference in peptides with differential urinary abundance between patients who received a graft for virus related liver disease vs. all other causes of liver disease was observed.

CONCLUSION:

Urinary peptidome analysis before liver transplantation failed to identify a peptide pattern associated with the development of CKD at 6 months after liver transplantation.

KEYWORDS:

chronic kidney disease; liver transplantation; proteome analysis; urine; viral hepatitis

PMID:
25515948
DOI:
10.1111/liv.12763
[Indexed for MEDLINE]

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