Format

Send to

Choose Destination
Invest Ophthalmol Vis Sci. 2014 Dec 16;56(1):346-52. doi: 10.1167/iovs.14-15416.

Levels of blood CD1c+ mDC1 and CD1chi mDC1 subpopulation reflect disease activity in noninfectious uveitis.

Author information

1
Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States.
2
Department of Clinical Sciences, University of Bristol, Bristol, United Kingdom.

Abstract

PURPOSE:

Myeloid dendritic cells (mDCs) play an important role in autoimmune diseases. However, the role of blood CD1c(+) myeloid dendritic cells 1 (mDC1s), the subset of human blood mDCs, is not well understood in noninfectious uveitis.

METHODS:

Fresh peripheral blood samples from human noninfectious uveitis patients (n = 32) and healthy controls (HCs) (n = 64) were stained with FITC-Lineage 1 (Lin1), PERCP-HLADR, and PE-CD1c antibodies. The levels of mDC1 were quantified by using flow cytometric analysis. Longitudinal data from patients (n = 16) were analyzed to correlate the levels of mDC1 with disease activity.

RESULTS:

Blood CD1c(+) mDC1 and its subpopulation, CD1c(hi) mDC1, were increased in uveitis patients compared with HCs. Longitudinal data demonstrated that both the CD1c(+) mDC1 and CD1c(hi) mDC1 subpopulation reflected a dynamic change in clinical uveitis activity: CD1c expression was increased in active uveitis but decreased when uveitis became inactive.

CONCLUSIONS:

Given these observations, an alteration in blood CD1c(+) mDC1 and the CD1c(hi) mDC1 subpopulation could be a potential biomarker to monitor clinical uveitis activity within patients.

KEYWORDS:

CD1c+ mDC1; CD1chi mDC1 subpopulation; autoimmune disease; noninfectious uveitis

PMID:
25515573
PMCID:
PMC4294291
DOI:
10.1167/iovs.14-15416
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center