Format

Send to

Choose Destination
Proteomics. 2015 Apr;15(7):1224-9. doi: 10.1002/pmic.201400436. Epub 2015 Feb 5.

Gel-aided sample preparation (GASP)--a simplified method for gel-assisted proteomic sample generation from protein extracts and intact cells.

Author information

1
TDI Mass Spectrometry Laboratory, Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.

Abstract

We describe a "gel-assisted" proteomic sample preparation method for MS analysis. Solubilized protein extracts or intact cells are copolymerized with acrylamide, facilitating denaturation, reduction, quantitative cysteine alkylation, and matrix formation. Gel-aided sample preparation has been optimized to be highly flexible, scalable, and to allow reproducible sample generation from 50 cells to milligrams of protein extracts. This methodology is fast, sensitive, easy-to-use on a wide range of sample types, and accessible to nonspecialists.

KEYWORDS:

Bottom-up proteomics; Comparative proteomics; Gel-assisted sample preparation; Sample preparation; Technology

PMID:
25515006
PMCID:
PMC4409837
DOI:
10.1002/pmic.201400436
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center