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Biochem Biophys Res Commun. 2015 Jan 16;456(3):815-20. doi: 10.1016/j.bbrc.2014.12.023. Epub 2014 Dec 13.

Triptolide inhibits proliferation of Epstein-Barr virus-positive B lymphocytes by down-regulating expression of a viral protein LMP1.

Author information

1
Department of Pathogen Biology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, PR China.
2
Department of Pathology and Physiology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, PR China.
3
Department of Pathogen Biology, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, PR China; State Key Laboratory of Virology, Wuhan University, Wuhan 430072, PR China. Electronic address: xsun6@whu.edu.cn.

Abstract

Epstein-Barr virus (EBV) infects various types of cells and mainly establishes latent infection in B lymphocytes. The viral latent membrane protein 1 (LMP1) plays important roles in transformation and proliferation of B lymphocytes infected with EBV. Triptolide is a compound of Tripterygium extracts, showing anti-inflammatory, immunosuppressive, and anti-cancer activities. In this study, it is determined whether triptolide inhibits proliferation of Epstein-Barr virus-positive B lymphocytes. The CCK-8 assays were performed to examine cell viabilities of EBV-positive B95-8 and P3HR-1 cells treated by triptolide. The mRNA and protein levels of LMP1 were examined by real time-PCR and Western blotting, respectively. The activities of two LMP1 promoters (ED-L1 and TR-L1) were determined by Dual luciferase reportor assay. The results showed that triptolide inhibited the cell viability of EBV-positive B lymphocytes, and the over-expression of LMP1 attenuated this inhibitory effect. Triptolide decreased the LMP1 expression and transcriptional levels in EBV-positive B cells. The activity of LMP1 promoter ED-L1 in type III latent infection was strongly suppressed by triptolide treatment. In addition, triptolide strongly reduced growth of B95-8 induced B lymphoma in BALB/c nude mice. These results suggest that triptolide decreases proliferation of EBV-induced B lymphocytes possibly by a mechanism related to down-regulation of the LMP1 expression.

KEYWORDS:

B lymphocytes; EBV; ED-L1; LMP1; Triptolide

PMID:
25511707
DOI:
10.1016/j.bbrc.2014.12.023
[Indexed for MEDLINE]

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