Abstract
Human β-defensin-2(HBD-2) is one of the two major vertebrate antimicrobial peptide families (α and β), which is highly expressed by proinflammatory induction in the lung and exhibit broad-spectrum antimicrobial activity. We observed that IL-22 receptors high expressed on the membrane of A549 cells; HBD-2 mRNA was expressed in a time- and concentration-dependent manners in A549 cells when treated with IL-22; further studies demonstrated that HBD-2 expression was attenuated by AG490, but to JSH-23, inhibitors of p-STAT3 DNA binding and NF-κB/p65 subunit nuclear translocation, respectively. These results support that IL-22-mediated signalling pathway of HBD-2 gene expression involved STAT3 but not NF-κB in human alveolar epithelium. These findings provide a new insight into how IL-22 may play an important link between innate and adaptive immunity, thereby anti-infection locally in the alveolar epithelium.
MeSH terms
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Cell Line
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DNA-Binding Proteins / antagonists & inhibitors
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Epithelial Cells / metabolism
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Humans
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Inflammation / immunology
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Interleukin-22
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Interleukins / metabolism*
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Phenylenediamines / pharmacology
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Pulmonary Alveoli / immunology*
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RNA, Messenger / biosynthesis
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Receptors, Interleukin / metabolism
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Respiratory Mucosa / immunology*
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STAT3 Transcription Factor / antagonists & inhibitors
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STAT3 Transcription Factor / metabolism*
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Signal Transduction
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Transcription Factor RelA / antagonists & inhibitors
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Transcription Factor RelA / metabolism
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Tyrphostins / pharmacology
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beta-Defensins / biosynthesis
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beta-Defensins / metabolism*
Substances
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4-methyl-N1-(3-phenylpropyl)benzene-1,2-diamine
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DEFB4A protein, human
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DNA-Binding Proteins
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Interleukins
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Phenylenediamines
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RELA protein, human
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RNA, Messenger
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Receptors, Interleukin
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STAT3 Transcription Factor
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STAT3 protein, human
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Transcription Factor RelA
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Tyrphostins
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alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide
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beta-Defensins