Format

Send to

Choose Destination
Nucleic Acids Res. 1989 Aug 25;17(16):6473-84.

Identification of sequences regulating the transcription of a Dictyostelium gene selectively expressed in prespore cells.

Author information

1
ICRF, Clare Hall Laboratory, South Mimms, Herts, UK.

Abstract

There has been considerable debate about the relative contributions of transcriptional and post-transcriptional mechanisms to the regulation of prespore gene expression in Dictyostelium. We have determined the DNA sequence upstream of D19, the Dictyostelium gene encoding PsA, a prespore-specific, cell surface protein of unknown function. Our analysis of gene fusions, in which D19 upstream sequences are placed adjacent to a heterologous reporter gene, indicates that transcriptional signals alone are sufficient for the correct temporal and cell-type specific expression of this gene. We also show that the 5' and 3' boundaries of the minimal sequences necessary for correct developmental regulation lie within the region 338 to 122 nucleotides upstream of the start site of transcription but that flanking sequences seem to be necessary for optimal expression.

PMID:
2550894
PMCID:
PMC318342
DOI:
10.1093/nar/17.16.6473
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center