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Mol Metab. 2014 Sep 16;3(9):834-47. doi: 10.1016/j.molmet.2014.09.003. eCollection 2014 Dec.

The ω6-fatty acid, arachidonic acid, regulates the conversion of white to brite adipocyte through a prostaglandin/calcium mediated pathway.

Author information

1
Univ. Nice Sophia Antipolis, iBV, UMR 7277, 06100 Nice, France ; CNRS, iBV, UMR 7277, 06100 Nice, France ; Inserm, iBV, U1091, 06100 Nice, France.
2
Lipidomic Core Facility, Metatoul Platform, France ; INSERM, UMR1048, Obesity Research Laboratory, Institute of Metabolic and Cardiovascular Diseases, Toulouse, France ; University of Toulouse, UMR1048, Paul Sabatier University, Toulouse, France.
3
Joint Division Molecular Metabolic Control, Alliance and Network Aging Research, German Cancer Research Center (DKFZ), Center for Molecular Biology (ZMBH) and University Hospital, Heidelberg University, Heidelberg, Germany.
4
Univ. Nice Sophia Antipolis, LP2M, UMR 7370, 06100 Nice, France ; UMR 7370, CNRS-LP2M, 06100 Nice, France.
5
INSERM, UMR1048, Obesity Research Laboratory, Institute of Metabolic and Cardiovascular Diseases, Toulouse, France ; University of Toulouse, UMR1048, Paul Sabatier University, Toulouse, France ; Toulouse University Hospitals, Department of Clinical Biochemistry, Toulouse, France.

Abstract

OBJECTIVE:

Brite adipocytes are inducible energy-dissipating cells expressing UCP1 which appear within white adipose tissue of healthy adult individuals. Recruitment of these cells represents a potential strategy to fight obesity and associated diseases.

METHODS/RESULTS:

Using human Multipotent Adipose-Derived Stem cells, able to convert into brite adipocytes, we show that arachidonic acid strongly inhibits brite adipocyte formation via a cyclooxygenase pathway leading to secretion of PGE2 and PGF2α. Both prostaglandins induce an oscillatory Ca(++) signaling coupled to ERK pathway and trigger a decrease in UCP1 expression and in oxygen consumption without altering mitochondriogenesis. In mice fed a standard diet supplemented with ω6 arachidonic acid, PGF2α and PGE2 amounts are increased in subcutaneous white adipose tissue and associated with a decrease in the recruitment of brite adipocytes.

CONCLUSION:

Our results suggest that dietary excess of ω6 polyunsaturated fatty acids present in Western diets, may also favor obesity by preventing the "browning" process to take place.

KEYWORDS:

Calcium oscillation; PGE2; PGF2α; PGI2; Polyunsaturated fatty acids; UCP1

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