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J Immunol. 2015 Jan 15;194(2):522-30. doi: 10.4049/jimmunol.1400626. Epub 2014 Dec 10.

Antigen recognition in the islets changes with progression of autoimmune islet infiltration.

Author information

1
Department of Biomedical Research, National Jewish Health, Denver, CO 80206; Department of Immunology and Microbiology, University of Colorado School of Medicine, Denver, CO 80206;
2
Department of Pathology, University of California San Francisco, San Francisco, CA 94143; and.
3
Department of Surgery, University of California San Francisco, San Francisco, CA 94143.
4
Department of Biomedical Research, National Jewish Health, Denver, CO 80206;
5
Department of Biomedical Research, National Jewish Health, Denver, CO 80206; Department of Immunology and Microbiology, University of Colorado School of Medicine, Denver, CO 80206; FriedmanR@NJHealth.org.

Abstract

In type 1 diabetes, the pancreatic islets are an important site for therapeutic intervention because immune infiltration of the islets is well established at diagnosis. Therefore, understanding the events that underlie the continued progression of the autoimmune response and islet destruction is critical. Islet infiltration and destruction is an asynchronous process, making it important to analyze the disease process on a single islet basis. To understand how T cell stimulation evolves through the process of islet infiltration, we analyzed the dynamics of T cell movement and interactions within individual islets of spontaneously autoimmune NOD mice. Using both intravital and explanted two-photon islet imaging, we defined a correlation between increased islet infiltration and increased T cell motility. Early T cell arrest was Ag dependent and due, at least in part, to Ag recognition through sustained interactions with CD11c(+) APCs. As islet infiltration progressed, T cell motility became Ag independent, with a loss of T cell arrest and sustained interactions with CD11c(+) APCs. These studies suggest that the autoimmune T cell response in the islets may be temporarily dampened during the course of islet infiltration and disease progression.

PMID:
25505281
PMCID:
PMC4282963
DOI:
10.4049/jimmunol.1400626
[Indexed for MEDLINE]
Free PMC Article

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