Format

Send to

Choose Destination
Microbiology. 2015 Feb;161(Pt 2):411-21. doi: 10.1099/mic.0.000010. Epub 2014 Dec 10.

Streptococcus gordonii comCDE (competence) operon modulates biofilm formation with Candida albicans.

Author information

1
School of Oral and Dental Sciences, University of Bristol, Lower Maudlin Street, Bristol BS1 2LY, UK.
2
School of Oral and Dental Sciences, University of Bristol, Lower Maudlin Street, Bristol BS1 2LY, UK School of Biochemistry, University of Bristol, University Walk, Bristol BS8 1TD, UK.
3
School of Biochemistry, University of Bristol, University Walk, Bristol BS8 1TD, UK.
4
Department of Oral Biology, University at Buffalo, 223 Foster Hall, Buffalo, NY 14214, USA.
5
Center for Oral Health and Systemic Disease, University of Louisville School of Dentistry, 501 South Preston Street, Louisville, KY 40202, USA.
6
School of Oral and Dental Sciences, University of Bristol, Lower Maudlin Street, Bristol BS1 2LY, UK angela.nobbs@bristol.ac.uk.

Abstract

Candida albicans is a pleiomorphic fungus that forms mixed species biofilms with Streptococcus gordonii, an early colonizer of oral cavity surfaces. Activation of quorum sensing (QS; intercellular signalling) promotes monospecies biofilm development by these micro-organisms, but the role of QS in mixed species communities is not understood. The comCDE genes in S. gordonii encode a sensor-regulator system (ComDE), which is activated by the comC gene product (CSP, competence stimulating peptide) and modulates expression of QS-regulated genes. Dual species biofilms of S. gordonii ΔcomCDE or ΔcomC mutants with C. albicans showed increased biomass compared to biofilms of S. gordonii DL1 wild-type with C. albicans. The ΔcomCDE mutant dual species biofilms in particular contained more extracellular DNA (eDNA), and could be dispersed with DNase I or protease treatment. Exogenous CSP complemented the S. gordonii ΔcomC transformation deficiency, as well as the ΔcomC-C. albicans biofilm phenotype. Purified CSP did not affect C. albicans hyphal filament formation but inhibited monospecies biofilm formation by C. albicans. The results suggest that the S. gordonii comCDE QS-system modulates the production of eDNA and the incorporation of C. albicans into dual species biofilms.

PMID:
25505189
PMCID:
PMC4314648
DOI:
10.1099/mic.0.000010
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Ingenta plc Icon for PubMed Central
Loading ...
Support Center