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Small. 2015 Mar 25;11(12):1396-401. doi: 10.1002/smll.201402508. Epub 2014 Dec 12.

Compact halo-ligand-conjugated quantum dots for multicolored single-molecule imaging of overcrowding GPCR proteins on cell membranes.

Author information

1
Laboratory for Nano-Bio Probes, Quantitative Biology Center, Riken, Suita, 565-0874, Osaka, Japan.

Abstract

To detect single molecules within the optical diffraction limit (< ca. 200 nm), a multicolored imaging technique is developed using Halo-ligand conjugated quantum dots (Halo-QDs; <6 nm in diameter). Using three types of Halo-QDs, multicolored single-molecule fluorescence imaging of GPCR proteins in Dictyostelium cells is achieved.

KEYWORDS:

fluorescence; halo tags; imaging; membranes; proteins; quantum dots; single-molecule studies

PMID:
25504902
DOI:
10.1002/smll.201402508
[Indexed for MEDLINE]

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