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Plant Physiol Biochem. 2015 Jan;86:174-180. doi: 10.1016/j.plaphy.2014.12.004. Epub 2014 Dec 4.

Environmental cues induce changes of steviol glycosides contents and transcription of corresponding biosynthetic genes in Stevia rebaudiana.

Author information

1
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; Institute of Botany, Jiangsu Province and the Chinese Academy of Sciences, Nanjing 210014, China.
2
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; Institute of Botany, Jiangsu Province and the Chinese Academy of Sciences, Nanjing 210014, China. Electronic address: hsz1959@163.com.
3
Experimental Teaching Center of Ecological Environment of Jiangxi Province, Department of Landscape Architecture, Jiangxi University of Finance and Economics, Nanchang 330032, China.
4
Institute of Botany, Jiangsu Province and the Chinese Academy of Sciences, Nanjing 210014, China.

Abstract

Plant growth and secondary metabolism are commonly regulated by external cues such as light, temperature and water availability. In this study, the influences of low and high temperatures, dehydration, photoperiods, and different growing stages on the changes of steviol glycosides (SGs) contents and transcription levels of fifteen genes involved in SGs biosynthesis of Stevia rebaudiana Bertoni were examined using HPLC and RT-PCR. The observations showed that the transcript levels of all the fifteen genes were maximum under 25 °C treatment, and the transcription of SrDXS, SrDXR, SrMCT, SrCMK, SrMDS, SrHDS, SrHDR, SrIDI, SrGGDPS, SrCPPS1, SrUGT85C2 and SrUGT76G1 were restrained both in low temperature (15 °C) and high temperature (35 °C). Most genes in SGs biosynthesis pathway exhibited down-regulation in dehydration. To elucidate the effect of photoperiods, the plants were treated by different simulated photoperiods (8 L/16 D, 1 0L/14 D, 14 L/10 D and 16 L/8 D), but no significant transcription changes were observed. In the study of growing stages, there were evident changes of SGs contents, and the transcript levels of all the fifteen genes were minimal in fast growing period, and exhibited evident increase both in flower-bud appearing stage and flowering stage. The obtained results strongly suggest that the effect of environmental cues on steviol glycosides contents and transcription of corresponding biosynthetic genes in S. rebaudiana is significant. It is worth to study deeply.

KEYWORDS:

Environmental cues; Stevia rebaudiana Bertoni; Steviol glycosides; Transcription

PMID:
25500454
DOI:
10.1016/j.plaphy.2014.12.004
[Indexed for MEDLINE]

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