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Int J Biol Macromol. 2015 Mar;74:68-75. doi: 10.1016/j.ijbiomac.2014.11.043. Epub 2014 Dec 9.

Antioxidant and anti-inflammatory activities of porphyran isolated from discolored nori (Porphyra yezoensis).

Author information

1
Graduate School of Fisheries Science and Environmental Studies, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Nagasaki, Japan.
2
Korea University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon, Republic of Korea; Jeju center, Korea Basic Science Institute (KBSI), Jeju 690-756, Republic of Korea.
3
Korea University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon, Republic of Korea; Jeju center, Korea Basic Science Institute (KBSI), Jeju 690-756, Republic of Korea. Electronic address: dkim@kbsi.re.kr.
4
Graduate School of Fisheries Science and Environmental Studies, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Nagasaki, Japan; Division of Biochemistry, Faculty of Fisheries, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Nagasaki, Japan. Electronic address: t-oda@nagasaki-u.ac.jp.

Abstract

We found that discolored waste nori with no commercial value, contains much higher level of porphyran than normal nori that is a sheeted food stuff prepared from P. yezoensis used in sushi. Chemical analyses revealed that mean molecular mass of the porphyran prepared from discolored nori (dc-porphyran) was much lower than that of the porphyran from normal nori (n-porphyran). Dc-porphyran showed slightly greater scavenging activity toward superoxide anion and hydroxyl radical than n-porphyran. Dc-porphyran inhibited nitric oxide (NO) production in LPS-stimulated RAW264.7 cells through preventing the expression of inducible NO synthase, whereas no such activity was observed in n-porphyran. Since acid-hydrolyzed n-porphyran showed the inhibitory activity on NO production from LPS-stimulated RAW264.7 cells, the molecular size of porphyran was suggested to be a critical factor for the activity. Dc-porphyran was separated into 4 fractions (F1-F4) on DEAE-chromatography, and F1 showed the highest inhibitory effect on NO production from LPS-stimulated RAW264.7 cells. Our results indicate that discolored waste nori is useful as a source of porphyran with even better bioactivities than porphyran from normal nori.

KEYWORDS:

Anti-inflammatory activity; Antioxidant activity; Porphyra yezoensis

PMID:
25499893
DOI:
10.1016/j.ijbiomac.2014.11.043
[Indexed for MEDLINE]

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