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Carbohydr Polym. 2015 Mar 6;117:115-22. doi: 10.1016/j.carbpol.2014.09.039. Epub 2014 Sep 28.

Structural analyses and immunomodulatory properties of fructo-oligosaccharides from onion (Allium cepa).

Author information

1
Department of Biochemistry and Nutrition, CSIR-Central Food Technological Research Institute (CFTRI), Mysore, Karnataka, India.
2
Department of Meat and Marine Sciences, CSIR-Central Food Technological Research Institute, Mysore, Karnataka, India.
3
Department of Biochemistry and Nutrition, CSIR-Central Food Technological Research Institute (CFTRI), Mysore, Karnataka, India. Electronic address: venkatyp@yahoo.com.

Abstract

Onion (Allium cepa) is an immune-boosting food rich in fructans. The major aim of this study is to characterize and investigate the immunomodulatory properties of onion fructo-oligosaccharides (FOS). FOS was isolated from onion bulbs by hot 80% ethanol extraction (yield: ∼4.5 g/100 g fw) followed by gel permeation chromatography. NMR of onion FOS revealed unusual β-D-Glc terminal residue at the non-reducing end. TLC and ESI-MS analyses showed that onion FOS ranged from trisaccharides to hexasaccharides. Onion FOS (50 μg/mL) significantly increased (∼3-fold) the proliferation of mouse splenocytes/thymocytes vs. control. Further, onion FOS enhanced (∼2.5-fold) the production of nitric oxide by peritoneal exudates cells (PECs) from Wistar rats; intracellular free radicals production and phagocytic activity of isolated murine PECs were also augmented. Our structural and in vitro results indicate that onion FOS comprising of tri- to hexasaccharide units belongs to inulin-type fructans, and possess immunostimulatory activities towards murine lymphocytes and macrophages.

KEYWORDS:

Fructo-oligosaccharides; Immunomodulation; Inulin; Mitogenicity; Onion; Phagocytosis

PMID:
25498616
DOI:
10.1016/j.carbpol.2014.09.039
[Indexed for MEDLINE]

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