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J Pharm Biomed Anal. 2015 Feb;104:97-104. doi: 10.1016/j.jpba.2014.11.034. Epub 2014 Nov 24.

Ultrasound-assisted ionic liquid-based homogeneous liquid-liquid microextraction high-performance liquid chromatography for determination of tanshinones in Salvia miltiorrhiza Bge. root.

Author information

1
College of Chemistry, Jilin University, Changchun 130012, PR China; College of Chemistry and Life Science, Changchun University of Technology, Changchun 130012, PR China.
2
College of Chemistry, Jilin University, Changchun 130012, PR China.
3
College of Chemistry, Jilin University, Changchun 130012, PR China; College of Chemistry and Life Science, Changchun University of Technology, Changchun 130012, PR China. Electronic address: analchem@jlu.edu.cn.

Abstract

The ultrasound-assisted ionic liquid-based homogeneous liquid-liquid microextraction has been developed and applied to the extraction of four tanshinones, including dihydrotanshinone, tanshinone I, cryptotanshinone and tanshinone IIA in Salvia miltiorrhiza Bge. root. High performance liquid chromatography was applied to the separation and determination of the analytes. The ionic liquid was used as extraction solvent and target analytes were extracted with help of ultrasound. Then, ion-pairing agent was added into the sample solution, which resulted in the formation of water-insoluble ionic liquid in the solution. The phase separation was performed by centrifugation. The extraction, concentration and purification of target analytes were performed simultaneously. The experimental parameters, including type and volume of ionic liquid, sample amount, the size of sample particle, pH value of extraction medium, extraction temperature, extraction time, amount of ion-pairing agent and centrifuging time, were investigated and optimized. The calibration curves showed good linear relationship (r>0.9997). The limits of detection and quantification were in the range of 0.052-0.093 and 0.17-0.31 μg mL(-1), respectively. The recoveries were between 70.45% and 94.23% with relative standard deviations lower than 5.31%. The present method is free of volatile organic solvents, and represents lower expenditures of sample, extraction time and solvent, compared with UAE and HRE. There was no obvious difference in the extraction yields of active constitutions obtained by the three extraction methods.

KEYWORDS:

High-performance liquid chromatography; Ionic liquid; Salvia miltiorrhiza Bge. root; Tanshinones; Ultrasound-assisted ionic liquid-based homogeneous liquid–liquid microextraction

PMID:
25497891
DOI:
10.1016/j.jpba.2014.11.034
[Indexed for MEDLINE]

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