1. Parasitol Res. 1989;75(7):522-7.

A stereological study of the differentiation process in Trypanosoma cruzi.

Soares MJ(1), Souto-Padrón T, Bonaldo MC, Goldenberg S, de Souza W.

Author information: 
(1)Laboratório de Ultraestrutura Celular e Microscopia Eletrônica, Instituto de
Biofĭsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.

When epimastigote forms of Trypanosoma cruzi grown in a rich medium (LIT) are
transferred to a simple, chemically defined medium (TAU3AAG, containing Ca2+,
Mg2+, K+, Na+, L-proline, L-glutamate, and L-aspartate in phosphate buffer) they 
transform into trypomastigote forms. Morphometric analysis of transmission
electron micrographs of thin sections of parasites collected at different steps
of the transformation process showed that no changes occurred in the volume
density of mitochondria and cytoplasmic vacuoles. However, a significant increase
in the volume density of the kinetoplast DNA network as well as the lipid
inclusions and a decrease in that of the reservosome (a special type of endosome)
was observed. These observations suggest that during differentiation, T. cruzi
accumulates lipids and uses molecules contained in the reservosome as its main
energy source.


PMID: 2549536  [Indexed for MEDLINE]