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ACS Nano. 2014 Dec 23;8(12):12725-33. doi: 10.1021/nn505821y. Epub 2014 Dec 12.

Imaging and sizing of single DNA molecules on a mobile phone.

Author information

1
Electrical Engineering Department, University of California , Los Angeles, California 90095, United States.

Abstract

DNA imaging techniques using optical microscopy have found numerous applications in biology, chemistry and physics and are based on relatively expensive, bulky and complicated set-ups that limit their use to advanced laboratory settings. Here we demonstrate imaging and length quantification of single molecule DNA strands using a compact, lightweight and cost-effective fluorescence microscope installed on a mobile phone. In addition to an optomechanical attachment that creates a high contrast dark-field imaging setup using an external lens, thin-film interference filters, a miniature dovetail stage and a laser-diode for oblique-angle excitation, we also created a computational framework and a mobile phone application connected to a server back-end for measurement of the lengths of individual DNA molecules that are labeled and stretched using disposable chips. Using this mobile phone platform, we imaged single DNA molecules of various lengths to demonstrate a sizing accuracy of <1 kilobase-pairs (kbp) for 10 kbp and longer DNA samples imaged over a field-of-view of ∼2 mm2.

KEYWORDS:

DNA imaging; DNA sizing; cell phone microscopy; fluorescence imaging; mobile health; point-of-care

PMID:
25494442
DOI:
10.1021/nn505821y
[Indexed for MEDLINE]

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