Format

Send to

Choose Destination
Angew Chem Int Ed Engl. 2015 Jan 26;54(5):1587-90. doi: 10.1002/anie.201410647. Epub 2014 Dec 9.

High-resolution N(6) -methyladenosine (m(6) A) map using photo-crosslinking-assisted m(6) A sequencing.

Author information

1
Department of Chemistry, Institute for Biophysical Dynamics, Howard Hughes Medical Institute, The University of Chicago, 929 East 57th Street, Chicago, IL 60637 (USA) http://he-group.uchicago.edu.

Abstract

N(6) -methyladenosine (m(6) A) is an abundant internal modification in eukaryotic mRNA and plays regulatory roles in mRNA metabolism. However, methods to precisely locate the m(6) A modification remain limited. We present here a photo-crosslinking-assisted m(6) A sequencing strategy (PA-m(6) A-seq) to more accurately define sites with m(6) A modification. Using this strategy, we obtained a high-resolution map of m(6) A in a human transcriptome. The map resembles the general distribution pattern observed previously, and reveals new m(6) A sites at base resolution. Our results provide insight into the relationship between the methylation regions and the binding sites of RNA-binding proteins.

KEYWORDS:

N6-methyladenosine; RNA modification; photo-crosslinking; transcriptome sequencing

PMID:
25491922
PMCID:
PMC4396828
DOI:
10.1002/anie.201410647
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center