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Biochem Biophys Res Commun. 2015 Jan 9;456(2):586-90. doi: 10.1016/j.bbrc.2014.11.114. Epub 2014 Dec 6.

Umami-bitter interactions: the suppression of bitterness by umami peptides via human bitter taste receptor.

Author information

1
Research Group of Food Functionality, Korea Food Research Institute, Bundang-gu, Sungnam-si, Gyeonggi-do, Republic of Korea.
2
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
3
Research Group of Food Functionality, Korea Food Research Institute, Bundang-gu, Sungnam-si, Gyeonggi-do, Republic of Korea. Electronic address: mrrhyu@kfri.re.kr.

Abstract

Taste-taste interactions often showed in human psychophysical studies. Considering that each tastant in foodstuffs individually stimulates its responsible gustatory systems to elicit relevant taste modalities, taste-taste interaction should be performed in taste receptor cell-based assay. While umami substances have been proposed to suppress the bitterness of various chemicals in human sensory evaluation, the bitter-umami interaction has not been explored in bitter taste receptors, TAS2Rs. We investigated umami-bitter taste interactions by presenting umami peptides with bitter substance (salicin) on Ca(2+)-flux signaling assay using hTAS2R16-expressing cells. Five representative umami peptides (Glu-Asp, Glu-Glu, Glu-Ser, Asp-Glu-Ser, and Glu-Gly-Ser) derived from soybean markedly attenuated the salicin-induced intracellular calcium influx in a time-dependent manner, respectively, while Gly-Gly, a tasteless peptide did not. The efficacies of Glu-Glu suppressing salicin-induced activation of hTAS2R16 were higher than that of probenecid, a specific antagonist of hTAS2R16. According to Ca(2+)-flux signaling assay using the mixtures of salicin and umami peptides, all five umami peptides suppressed salicin-induced intracellular calcium influx in a noncompetitive manner. These results may provide evidence that umami peptides suppress bitter taste via bitter taste receptor(s). This is the first report which defines the interaction between bitter and umami taste in taste receptor level.

KEYWORDS:

Bitter taste receptor; Bitterness suppressing effects; Glutamyl peptides; Umami–bitter interaction; hTAS2R16

PMID:
25490385
DOI:
10.1016/j.bbrc.2014.11.114
[Indexed for MEDLINE]

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