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J Biomed Opt. 2014 Dec;19(12):126006. doi: 10.1117/1.JBO.19.12.126006.

Gaussian beam in two-photon fluorescence imaging of rat brain microvessel.

Author information

1
The City College of New York, Department of Biomedical Engineering, 160 Convent Avenue, New York, New York 10031, United StatesbThe City College of New York, Department of Biology, 160 Convent Avenue, New York, New York 10031, United StatescThe City Colle.
2
The City College of New York, Department of Biology, 160 Convent Avenue, New York, New York 10031, United StatescThe City College of New York, Institute for Ultrafast Spectroscopy and Lasers and Departments of Electrical Engineering and Physics, 160 Conve.
3
The City College of New York, Institute for Ultrafast Spectroscopy and Lasers and Departments of Electrical Engineering and Physics, 160 Convent Avenue, New York, New York 10031, United States.

Abstract

The critical optical properties of a Gaussian laser beam in two-photon or multiphoton fluorescence imaging, including the beam spot size, depth of focus, and intensity profile, are investigated for spatially locating nanoscale solutes in and surrounding the microvessels of rat brain.

PMID:
25490048
PMCID:
PMC4260768
DOI:
10.1117/1.JBO.19.12.126006
[Indexed for MEDLINE]
Free PMC Article

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