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Int J Syst Evol Microbiol. 2015 Feb;65(Pt 2):656-62. doi: 10.1099/ijs.0.068395-0. Epub 2014 Dec 8.

Characterization of 17 strains belonging to the Mycobacterium simiae complex and description of Mycobacterium paraense sp. nov.

Author information

1
Bacteriology and Mycology Section, Evandro Chagas Institute, Pará, Brazil.
2
NGS Facility, Laboratory of Biomolecular Sequence and Structure Analysis for Health, Centre for Integrative Biology, University of Trento, Italy.
3
Microbiology and Virology Unit, Policlinico University Hospital, Modena, Italy.
4
Genetic Unit, Careggi University Hospital, Florence, Italy.
5
Emerging Bacterial Pathogens Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy tortoli.enrico@hsr.it.

Abstract

Fourteen mycobacterial strains isolated from pulmonary samples of independent patients in the state of Pará (Brazil), and three strains isolated in Italy, were characterized using a polyphasic approach. Thorough genetic investigation, including whole-genome sequencing, demonstrated that the strains belong to the M. simiae complex, being most closely related to Mycobacterium interjectum. For 14 of the strains, evidence emerged supporting their inclusion in a previously unreported species of the genus Mycobacterium, for which the name Mycobacterium paraense sp. nov. is proposed (type strain, IEC26(T) = DSM 46749(T) = CCUG 66121(T)). The novel species is characterized by slow growth, unpigmented or pale yellow scotochromogenic colonies, and a HPLC mycolic acid profile different from other known mycobacteria. In different genetic regions, high sequence microheterogeneity was detected.

PMID:
25487637
DOI:
10.1099/ijs.0.068395-0
[Indexed for MEDLINE]

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