Format

Send to

Choose Destination
J Struct Biol. 2015 Feb;189(2):114-22. doi: 10.1016/j.jsb.2014.11.010. Epub 2014 Dec 6.

Semi-automated selection of cryo-EM particles in RELION-1.3.

Author information

1
MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Francis Crick Avenue, Cambridge CB2 0QH, UK. Electronic address: scheres@mrc-lmb.cam.ac.uk.

Abstract

The selection of particles suitable for high-resolution cryo-EM structure determination from noisy micrographs may represent a tedious and time-consuming step. Here, a semi-automated particle selection procedure is presented that has been implemented within the open-source software RELION. At the heart of the procedure lies a fully CTF-corrected template-based picking algorithm, which is supplemented by a fast sorting algorithm and reference-free 2D class averaging to remove false positives. With only limited user-interaction, the proposed procedure yields results that are comparable to manual particle selection. Together with an improved graphical user interface, these developments further contribute to turning RELION from a stand-alone refinement program into a convenient image processing pipeline for the entire single-particle approach.

KEYWORDS:

Automated particle picking; Electron cryo-microscopy; Single-particle analysis

PMID:
25486611
PMCID:
PMC4318617
DOI:
10.1016/j.jsb.2014.11.010
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center