Format

Send to

Choose Destination
Nat Methods. 2015 Feb;12(2):137-9. doi: 10.1038/nmeth.3207. Epub 2014 Dec 8.

Genetic targeting of chemical indicators in vivo.

Author information

1
1] Mouse Biology Unit, European Molecular Biology Laboratory (EMBL), Monterotondo, Italy. [2] Molecular Medicine Partnership Unit (MMPU), Heidelberg, Germany.
2
Mouse Biology Unit, European Molecular Biology Laboratory (EMBL), Monterotondo, Italy.
3
1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2] National Centre of Competence of Research in Chemical Biology, Lausanne, Switzerland.

Abstract

Fluorescent protein reporters have become the mainstay for tracing cellular circuitry in vivo but are limited in their versatility. Here we generated Cre-dependent reporter mice expressing the Snap-tag to target synthetic indicators to cells. Snap-tag labeling worked efficiently and selectively in vivo, allowing for both the manipulation of behavior and monitoring of cellular fluorescence from the same reporter.

PMID:
25486061
DOI:
10.1038/nmeth.3207
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center