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Nat Biotechnol. 2015 Mar;33(3):296-300. doi: 10.1038/nbt.3103. Epub 2014 Dec 8.

MinION nanopore sequencing identifies the position and structure of a bacterial antibiotic resistance island.

Author information

1
Gastrointestinal Bacteria Reference Unit, Public Health England, Colindale, London, UK.
2
1] Department of Biomedical Sciences, University of Sassari, Sassari, Italy. [2] Department of Infection and Immunity, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia.
3
National Reference Centre for Salmonellae and other Enterics, Robert Koch Institute, Wernigerode, Germany.
4
Norwich Medical School, University of East Anglia, Norwich, UK.

Abstract

Short-read, high-throughput sequencing technology cannot identify the chromosomal position of repetitive insertion sequences that typically flank horizontally acquired genes such as bacterial virulence genes and antibiotic resistance genes. The MinION nanopore sequencer can produce long sequencing reads on a device similar in size to a USB memory stick. Here we apply a MinION sequencer to resolve the structure and chromosomal insertion site of a composite antibiotic resistance island in Salmonella Typhi Haplotype 58. Nanopore sequencing data from a single 18-h run was used to create a scaffold for an assembly generated from short-read Illumina data. Our results demonstrate the potential of the MinION device in clinical laboratories to fully characterize the epidemic spread of bacterial pathogens.

PMID:
25485618
DOI:
10.1038/nbt.3103
[Indexed for MEDLINE]

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