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Nat Biotechnol. 2015 Jan;33(1):64-72. doi: 10.1038/nbt.3071. Epub 2014 Dec 8.

Injectable, spontaneously assembling, inorganic scaffolds modulate immune cells in vivo and increase vaccine efficacy.

Author information

1
1] School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA. [2] The Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA. [3] School of Chemical Engineering, Sungkyunkwan University, Suwon, Korea.
2
1] School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA. [2] The Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA.
3
School of Chemical Engineering, Sungkyunkwan University, Suwon, Korea.
4
The Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA.
5
Dana-Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts, USA.

Abstract

Implanting materials in the body to program host immune cells is a promising alternative to transplantation of cells manipulated ex vivo to direct an immune response, but doing so requires a surgical procedure. Here we demonstrate that high-aspect-ratio, mesoporous silica rods (MSRs) injected with a needle spontaneously assemble in vivo to form macroporous structures that provide a 3D cellular microenvironment for host immune cells. In mice, substantial numbers of dendritic cells are recruited to the pores between the scaffold rods. The recruitment of dendritic cells and their subsequent homing to lymph nodes can be modulated by sustained release of inflammatory signals and adjuvants from the scaffold. Moreover, injection of an MSR-based vaccine formulation enhances systemic helper T cells TH1 and TH2 serum antibody and cytotoxic T-cell levels compared to bolus controls. These findings suggest that injectable MSRs may serve as a multifunctional vaccine platform to modulate host immune cell function and provoke adaptive immune responses.

PMID:
25485616
PMCID:
PMC4318563
DOI:
10.1038/nbt.3071
[Indexed for MEDLINE]
Free PMC Article

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