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Chem Commun (Camb). 2015 Feb 11;51(12):2208-24. doi: 10.1039/c4cc09218j.

Expanding the toolbox of organic chemists: directed evolution of P450 monooxygenases as catalysts in regio- and stereoselective oxidative hydroxylation.

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GlaxoSmithKline R&D Ltd, Medicines Research Centre, Gunnels Wood Road, Stevenage, Hertfordshire, UK.


Cytochrome P450 enzymes (CYPs) have been used for more than six decades as catalysts for the CH-activating oxidative hydroxylation of organic compounds with formation of added-value products. However, it has not been possible to control regio- and stereoselectivity in a general manner, which is necessary for wide (industrial) applications. Especially directed evolution as a Darwinian approach to protein engineering has changed the situation for the better during the last 3-4 years leading to extensive progress, which is summarized and analysed in this Feature Article.

[Indexed for MEDLINE]

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