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Neuron. 2014 Dec 17;84(6):1191-7. doi: 10.1016/j.neuron.2014.11.009. Epub 2014 Dec 4.

Pyramidal and stellate cell specificity of grid and border representations in layer 2 of medial entorhinal cortex.

Author information

1
Bernstein Center for Computational Neuroscience, Humboldt University of Berlin, Philippstr. 13, Haus 6, 10115 Berlin, Germany.
2
Bernstein Center for Computational Neuroscience, Humboldt University of Berlin, Philippstr. 13, Haus 6, 10115 Berlin, Germany. Electronic address: andrea.burgalossi@cin.uni-tuebingen.de.
3
Bernstein Center for Computational Neuroscience, Humboldt University of Berlin, Philippstr. 13, Haus 6, 10115 Berlin, Germany; Berlin School of Mind and Brain, Humboldt University of Berlin, Unter den Linden 6, 10099 Berlin, Germany.
4
Bernstein Center for Computational Neuroscience, Humboldt University of Berlin, Philippstr. 13, Haus 6, 10115 Berlin, Germany. Electronic address: michael.brecht@bccn-berlin.de.

Abstract

In medial entorhinal cortex, layer 2 principal cells divide into pyramidal neurons (mostly calbindin positive) and dentate gyrus-projecting stellate cells (mostly calbindin negative). We juxtacellularly labeled layer 2 neurons in freely moving animals, but small sample size prevented establishing unequivocal structure-function relationships. We show, however, that spike locking to theta oscillations allows assigning unidentified extracellular recordings to pyramidal and stellate cells with ∼83% and ∼89% specificity, respectively. In pooled anatomically identified and theta-locking-assigned recordings, nonspatial discharges dominated, and weakly hexagonal spatial discharges and head-direction selectivity were observed in both cell types. Clear grid discharges were rare and mostly classified as pyramids (19%, 19/99 putative pyramids versus 3%, 3/94 putative stellates). Most border cells were classified as stellate (11%, 10/94 putative stellates versus 1%, 1/99 putative pyramids). Our data suggest weakly theta-locked stellate border cells provide spatial input to dentate gyrus, whereas strongly theta-locked grid discharges occur mainly in hexagonally arranged pyramidal cell patches and do not feed into dentate gyrus.

PMID:
25482025
PMCID:
PMC4276741
DOI:
10.1016/j.neuron.2014.11.009
[Indexed for MEDLINE]
Free PMC Article

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