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Brain Res. 2015 Feb 9;1597:65-76. doi: 10.1016/j.brainres.2014.11.040. Epub 2014 Dec 4.

Nop2 is expressed during proliferation of neural stem cells and in adult mouse and human brain.

Author information

1
Laboratory for Stem Cells, Croatian Institute for Brain Research, School of Medicine, University of Zagreb, 10 000 Zagreb, Croatia. Electronic address: ninakosi@hiim.hr.
2
Laboratory for Stem Cells, Croatian Institute for Brain Research, School of Medicine, University of Zagreb, 10 000 Zagreb, Croatia; Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, University of Zagreb, 10 000 Zagreb, Croatia. Electronic address: ialic@vef.hr.
3
Laboratory for Stem Cells, Croatian Institute for Brain Research, School of Medicine, University of Zagreb, 10 000 Zagreb, Croatia. Electronic address: matea00@gmail.com.
4
Laboratory for Stem Cells, Croatian Institute for Brain Research, School of Medicine, University of Zagreb, 10 000 Zagreb, Croatia. Electronic address: nvrsaljko@yahoo.com.
5
Laboratory for Immunohistochemistry, Croatian Institute for Brain Research, School of Medicine, University of Zagreb, 10 000 Zagreb, Croatia. Electronic address: njovanov@hiim.hr.
6
Department of Biology of the Cell Nucleus, Institute of Molecular Genetics of the Academy of Sciences of the Czech Republic, Prague, Czech Republic. Electronic address: Margarita.Sobol@img.cas.cz.
7
Department of Biology of the Cell Nucleus, Institute of Molecular Genetics of the Academy of Sciences of the Czech Republic, Prague, Czech Republic. Electronic address: Anatoly.Philimonenko@img.cas.cz.
8
Department of Biology of the Cell Nucleus, Institute of Molecular Genetics of the Academy of Sciences of the Czech Republic, Prague, Czech Republic. Electronic address: Pavel.Hozak@img.cas.cz.
9
Laboratory for Stem Cells, Croatian Institute for Brain Research, School of Medicine, University of Zagreb, 10 000 Zagreb, Croatia; Laboratory for Neurogenetics and Genetics of Development, Croatian Institute for Brain Research, School of Medicine, University of Zagreb, 10 000 Zagreb, Croatia. Electronic address: srecko.gajovic@cmj.hr.
10
Laboratory for Stem Cells, Croatian Institute for Brain Research, School of Medicine, University of Zagreb, 10 000 Zagreb, Croatia; Laboratory for Neurogenetics and Genetics of Development, Croatian Institute for Brain Research, School of Medicine, University of Zagreb, 10 000 Zagreb, Croatia; Laboratory of Histology, Neuroanatomy and Neuropathology, Université Libre de Bruxelles, 1070 Bruxelles, Belgium. Electronic address: rpochet@ulb.ac.be.
11
Laboratory for Stem Cells, Croatian Institute for Brain Research, School of Medicine, University of Zagreb, 10 000 Zagreb, Croatia; Laboratory for Neurogenetics and Genetics of Development, Croatian Institute for Brain Research, School of Medicine, University of Zagreb, 10 000 Zagreb, Croatia. Electronic address: dinko.mitrecic@mef.hr.

Abstract

The nucleolar protein 2 gene encodes a protein specific for the nucleolus. It is assumed that it plays a role in the synthesis of ribosomes and regulation of the cell cycle. Due to its link to cell proliferation, higher expression of Nop2 indicates a worse tumor prognosis. In this work we used Nop2(gt1gaj) gene trap mouse strain. While lethality of homozygous animals suggested a vital role of this gene, heterozygous animals allowed the detection of expression of Nop2 in various tissues, including mouse brain. Histochemistry, immunohistochemistry and immunoelectron microscopy techniques, applied to a mature mouse brain, human brain and on mouse neural stem cells revealed expression of Nop2 in differentiating cells, including astrocytes, as well as in mature neurons. Nop2 was detected in various regions of mouse and human brain, mostly in large pyramidal neurons. In the human, Nop2 was strongly expressed in supragranular and infragranular layers of the somatosensory cortex and in layer III of the cingulate cortex. Also, Nop2 was detected in CA1 and the subiculum of the hippocampus. Subcellular analyses revealed predominant location of Nop2 within the dense fibrillar component of the nucleolus. To test if Nop2 expression correlates to cell proliferation occurring during tissue regeneration, we induced strokes in mice by middle cerebral artery occlusion. Two weeks after stroke, the number of Nop2/nestin double positive cells in the region affected by ischemia and the periventricular zone substantially increased. Our findings suggest a newly discovered role of Nop2 in both mature neurons and in cells possibly involved in the regeneration of nervous tissue.

KEYWORDS:

Brain; Cell cycle; Nop2; Nucleolus; Stem cells; Stroke

PMID:
25481415
DOI:
10.1016/j.brainres.2014.11.040
[Indexed for MEDLINE]
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