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J Gen Virol. 2015 Mar;96(Pt 3):507-12. doi: 10.1099/jgv.0.000002. Epub 2014 Dec 5.

Monoclonal antibodies specific for the capsid protein of chikungunya virus suitable for multiple applications.

Author information

1
Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, Queensland 4072, Australia.
2
Inflammation Biology, QIMR Berghofer Medical Research Institute, Queensland 4029, Australia.
3
Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, Queensland 4072, Australia School of Veterinary Science, University of Queensland, Gatton, Queensland 4343, Australia.
4
Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, Queensland 4072, Australia Inflammation Biology, QIMR Berghofer Medical Research Institute, Queensland 4029, Australia.
5
Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, Queensland 4072, Australia roy.hall@uq.edu.au.

Abstract

Chikungunya virus (CHIKV) is a mosquito-borne pathogen responsible for epidemics of debilitating arthritic disease. The recent outbreak (2004-2014) resulted in an estimated 1.4-6.5 million cases, with imported cases reported in nearly 40 countries. The development of CHIKV-specific diagnostics and research tools is thus highly desirable. Herein we describe the generation and characterization of the first mAbs specific for the capsid protein (CP) of CHIKV. The antibodies recognized isolates representing the major genotypes of CHIKV, as well as several other alphaviruses, and were reactive in a range of assays including ELISA, Western blot, immunofluorescence and immunohistochemistry (IHC). We have also used the anti-CP mAb 5.5G9 in IHC studies to show that capsid antigen is persistently expressed 30 days post-infection in cells with macrophage morphology in a mouse model of chronic CHIKV disease. These antibodies may thus represent useful tools for further research, including investigations into the structure and function of CHIKV CP, and as valuable reagents for CHIKV detection in a range of settings.

PMID:
25480927
DOI:
10.1099/jgv.0.000002
[Indexed for MEDLINE]

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