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Plant Cell. 2014 Dec;26(12):4918-32. doi: 10.1105/tpc.114.132118. Epub 2014 Dec 5.

RHON1 mediates a Rho-like activity for transcription termination in plastids of Arabidopsis thaliana.

Author information

1
Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
2
Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China University of Chinese Academy of Sciences, Beijing 100049, China.
3
Biozentrum der Ludwig-Maximilians-Universität, Plant Molecular Biology/Botany, 82152 Planegg-Martinsried, Germany.
4
Departments of Molecular Biology and Plant Biology, University of Geneva, 1211 Geneva, Switzerland.
5
Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China zhanglixin@ibcas.ac.cn.

Erratum in

Abstract

Although transcription termination is essential to generate functional RNAs, its underlying molecular mechanisms are still poorly understood in plastids of vascular plants. Here, we show that the RNA binding protein RHON1 participates in transcriptional termination of rbcL (encoding large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase) in Arabidopsis thaliana. Inactivation of RHON1 leads to enhanced rbcL read-through transcription and to aberrant accD (encoding β-subunit of the acetyl-CoA carboxylase) transcriptional initiation, which may result from inefficient transcription termination of rbcL. RHON1 can bind to the mRNA as well as to single-stranded DNA of rbcL, displays an RNA-dependent ATPase activity, and terminates transcription of rbcL in vitro. These results suggest that RHON1 terminates rbcL transcription using an ATP-driven mechanism similar to that of Rho of Escherichia coli. This RHON1-dependent transcription termination occurs in Arabidopsis but not in rice (Oryza sativa) and appears to reflect a fundamental difference between plastomes of dicotyledonous and monocotyledonous plants. Our results point to the importance and significance of plastid transcription termination and provide insights into its machinery in an evolutionary context.

PMID:
25480370
PMCID:
PMC4311204
DOI:
10.1105/tpc.114.132118
[Indexed for MEDLINE]
Free PMC Article

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