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Anal Chim Acta. 2015 Jan 7;854:140-4. doi: 10.1016/j.aca.2014.11.012. Epub 2014 Nov 11.

Whole-cell Gluconobacter oxydans biosensor for 2-phenylethanol biooxidation monitoring.

Author information

1
Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84538 Bratislava, Slovakia.
2
Axxence Slovakia s.r.o., Poštová 1, SK-81106 Bratislava, Slovakia.
3
Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84538 Bratislava, Slovakia. Electronic address: jaroslav.katrlik@savba.sk.

Abstract

A microbial biosensor for 2-phenylethanol (2-PE) based on the bacteria Gluconobacter oxydans was developed and applied in monitoring of a biotechnological process. The cells of G. oxydans were immobilized within a disposable polyelectrolyte complex gel membrane consisting of sodium alginate, cellulose sulphate and poly(methylene-co-guanidine) attached onto a miniaturized Clark oxygen electrode, forming whole cell amperometric biosensor. Measured changes in oxygen concentration were proportional to changes in 2-PE concentration. The biosensor sensitivity was 864 nA mM(-1) (RSD=6%), a detection limit of 1 μM, and the biosensor response towards 2-PE was linear in the range 0.02-0.70 mM. The biosensor preserved 93% of its initial sensitivity after 7h of continuous operation and exhibited excellent storage stability with loss of only 6% of initial sensitivity within two months, when stored at 4°C. The developed system was designed and successfully used for an off-line monitoring of whole course of 2-PE biooxidation process producing phenylacetic acid (PA) as industrially valuable aromatic compound. The biosensor measurement did not require the use of hazardous organic solvent. The biosensor response to 2-PE was not affected by interferences from PA and phenylacetaldehyde at concentrations present in real samples during the biotransformation and the results were in a very good agreement with those obtained via gas chromatography.

KEYWORDS:

2-Phenylethanol; Biosensor; Electrochemistry; Gluconobacter oxydans; Immobilization; Phenylacetic acid

PMID:
25479877
DOI:
10.1016/j.aca.2014.11.012
[Indexed for MEDLINE]

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