Format

Send to

Choose Destination
Science. 2015 Jan 23;347(6220):400-6. doi: 10.1126/science.1260668. Epub 2014 Dec 4.

T cell immunity. Functional heterogeneity of human memory CD4⁺ T cell clones primed by pathogens or vaccines.

Author information

1
Institute for Research in Biomedicine, Bellinzona, Università della Svizzera Italiana, Lugano, Switzerland. Institute of Microbiology, ETH Zürich, Zürich, Switzerland.
2
Institute for Research in Biomedicine, Bellinzona, Università della Svizzera Italiana, Lugano, Switzerland.
3
Division of Immunology, Netherlands Cancer Institute, Amsterdam, Netherlands.
4
Institute for Research in Biomedicine, Bellinzona, Università della Svizzera Italiana, Lugano, Switzerland. federica.sallusto@irb.usi.ch.

Abstract

Distinct types of CD4(+) T cells protect the host against different classes of pathogens. However, it is unclear whether a given pathogen induces a single type of polarized T cell. By combining antigenic stimulation and T cell receptor deep sequencing, we found that human pathogen- and vaccine-specific T helper 1 (T(H)1), T(H)2, and T(H)17 memory cells have different frequencies but comparable diversity and comprise not only clones polarized toward a single fate, but also clones whose progeny have acquired multiple fates. Single naïve T cells primed by a pathogen in vitro could also give rise to multiple fates. Our results unravel an unexpected degree of interclonal and intraclonal functional heterogeneity of the human T cell response and suggest that polarized responses result from preferential expansion rather than priming.

Comment in

PMID:
25477212
DOI:
10.1126/science.1260668
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center