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Mol Cells. 2015 Jan 31;38(1):51-7. doi: 10.14348/molcells.2015.2202. Epub 2014 Dec 4.

A role for peroxidasin PXN-1 in aspects of C. elegans development.

Author information

1
Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500-712, Korea.
2
Department of Biotechnology, West Bengal University of Technology, BF-142, Salt Lake City, Sector I, Kolkata 700-064, India.
3
Division of Biological Science and Technology, College of Science and Technology, Yonsei University, Wonju 220-710, Korea.
4
Department of Biology Education, College of Education, Chosun University, Gwangju 501-759, Korea.

Abstract

The Caenorhabditis elegans peroxidasins, PXN-1 and PXN-2, are extracellular peroxidases; pxn-2 is involved in muscle-epidermal attachment during embryonic morphogenesis and in specific axon guidance. Here we investigate potential roles of the other homologue of peroxidasin, pxn-1, in C. elegans. A pxn-1 deletion mutant showed high lethality under heat-stress conditions. Using a transcriptional GFP reporter, pxn-1 expression was observed in various tissues including neurons, muscles, and hypodermis. A translational fusion showed that PXN-1::GFP was secreted and localized in extracellular matrix, particularly along body wall muscles and pharyngeal muscles. Various neuronal developmental defects were observed in pxn-1 mutants and in pxn-1 over-expressing animals, including handedness, branching, breakage, tangling, and defasciculation. These results suggest that pxn-1, like other peroxidasins, plays an important role throughout development.

KEYWORDS:

C. elegans; heat stress; neuronal development; peroxidasins; pxn-1

PMID:
25475546
PMCID:
PMC4314122
DOI:
10.14348/molcells.2015.2202
[Indexed for MEDLINE]
Free PMC Article

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