Sci Rep. 2014 Dec 4;4:7315. doi: 10.1038/srep07315.

Multiple types of logic gates based on a single G-quadruplex DNA strand.

Guo Y¹, Zhou L², Xu L², Zhou X³, Hu J³, Pei R².

Author information

1: 1] Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Collaborative Innovation Center of Suzhou Nano Science and Technology, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123 (China) [2] Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry &Molecular Sciences, Wuhan University, Wuhan, 430072 (China).
2: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Collaborative Innovation Center of Suzhou Nano Science and Technology, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, 215123 (China).
3: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry &Molecular Sciences, Wuhan University, Wuhan, 430072 (China).

Abstract

In this work, we demonstrate the use of a single DNA strand and G-quadruplex-specific dye NMM as a label-free switch for the construction of series of basic logic gates (YES, NOT, OR, INHIBIT, NOR, AND). The simple GT-rich sequence could be used to interact with several molecules (K(+), thrombin, Hg(2+), and Pb(2+)) to form different structures that can be distinguished by the label-free dye NMM. Our study showed that a single G-qudruplex DNA strand can function as multiple types of one-input and two-input logic gates with different combinations of input molecules.