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Ann Clin Biochem. 2015 May;52(Pt 3):337-45. doi: 10.1177/0004563214564225. Epub 2014 Dec 2.

Comparison of Hevylite™ IgA and IgG assay with conventional techniques for the diagnosis and follow-up of plasma cell dyscrasia.

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Department of Molecular and Translational Medicine, Faculty of Medicine, University of Brescia, Brescia, Italy
Department of Molecular and Translational Medicine, Faculty of Medicine, University of Brescia, Brescia, Italy.



Heavy/light chain assay allows the characterization and quantification of immunoglobulin light chains bound to heavy chains for each Ig'k and Ig'λ immunoglobulin class, discriminating between the involved/uninvolved isotypes in plasma cell dyscrasia. The Ig'k/Ig'λ ratio (heavy/light chain ratio) enables to monitor the trend of monoclonal component during therapy and disease evolution.


In this study, we evaluate the impact of the heavy/light chain assay in monitoring multiple myeloma patients in comparison with conventional techniques.


Serum samples of 28 patients with IgG or IgA monoclonal component were collected for a mean of 109 days and analyzed. The heavy/light chain assay was compared with classical immunoglobulin quantification (Ig'Tot), serum immunofixation electrophoresis, serum protein electrophoresis, and serum-free light chains quantification. Serum samples from 30 healthy patients were used as control (polyclonal).


Heavy/light chain ratio and serum immunofixation electrophoresis were comparable in 86% of the cases, and free light chain ratio and heavy/light chain ratio in 71.8%. Heavy/light chain assay and Ig'Tot measurements showed a concentration-dependent agreement in monoclonal patients. The heavy/light chain assay was able to quantify the monoclonal component migrating in SPE β region: this occurred in 10% of our IgG and 50% of our IgA patients.


The concordance scores indicate that heavy/light chain and Ig'Tot assays show differences at high monoclonal component values. The heavy/light chain ratio, serum immunofixation electrophoresis, and free light chain ratio showed partial concordance. Our study confirmed that, in the context of heavy/light chain assay, heavy/light chain Ig'k and Ig'λ absolute values and heavy/light chain ratio are both important tools to monitor the presence of monoclonal component that are difficult to be identified in SPE.


Heavy/light chain assay; immunoglobulin; monoclonal component quantification; plasma cell dyscrasia

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