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Chem Commun (Camb). 2015 Jan 21;51(6):1135-8. doi: 10.1039/c4cc08233h.

Capture and Release (CaR): a simplified procedure for one-tube isolation and concentration of single-stranded DNA during SELEX.

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1
Institute for Exp. Haematology and Transfusion Medicine, University of Bonn Medical Centre, Bonn, Germany. jens.mueller@ukb.uni-bonn.de.

Abstract

Short biotinylated oligodeoxynucleotides immobilized on streptavidin-coated magnetic beads allow for convenient and rapid purification of single-stranded oligodeoxynucleotides from crude asymmetric PCR mixtures, facilitating the selection of DNA aptamers.

PMID:
25466441
DOI:
10.1039/c4cc08233h
[Indexed for MEDLINE]
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