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Food Chem. 2015 Apr 15;173:741-8. doi: 10.1016/j.foodchem.2014.10.067. Epub 2014 Oct 25.

Tucumã fruit extracts (Astrocaryum aculeatum Meyer) decrease cytotoxic effects of hydrogen peroxide on human lymphocytes.

Author information

1
Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Av. Roraima 1000, Prédio 21, 97105-900 Santa Maria, RS, Brazil; Centro Universitário Franciscano, Rua dos Andradas, 1614, 97010-032 Santa Maria, RS, Brazil; Laboratório de Biogenômica, Departamento de Morfologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Av. Roraima, 1000 Prédio 19, 97105-900 Santa Maria, RS, Brazil. Electronic address: sagrillomr@yahoo.com.br.
2
Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Av. Roraima 1000, Prédio 21, 97105-900 Santa Maria, RS, Brazil; Laboratório de Biogenômica, Departamento de Morfologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Av. Roraima, 1000 Prédio 19, 97105-900 Santa Maria, RS, Brazil.
3
Laboratório de Biogenômica, Departamento de Morfologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Av. Roraima, 1000 Prédio 19, 97105-900 Santa Maria, RS, Brazil.
4
Universidade Aberta da Terceira Idade, Universidade do Estado do Amazonas, Amazonas, Brazil.
5
Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Av. Roraima 1000, Prédio 21, 97105-900 Santa Maria, RS, Brazil; Laboratório de Biogenômica, Departamento de Morfologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Av. Roraima, 1000 Prédio 19, 97105-900 Santa Maria, RS, Brazil; Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Av. Roraima 1000, Prédio 18, 97105-900 Santa Maria, RS, Brazil.

Abstract

This study quantifies the bioactive molecules in and determines the in vitro protective effect of ethanolic extracts isolated from the peel and pulp of tucumã (Astrocaryum aculeatum, Mart.), an Amazonian fruit rich in carotenoids. The cytoprotective effect of tucumã was evaluated in lymphocyte cultures exposed to H2O2 using spectrophotometric, fluorimetric, and immunoassay assays. The results confirmed that tucumã pulp extract is rich in β-carotene and quercetin, as previously described in the literature. However, high levels of these compounds were also found in tucumã peel extract. The extracts also contained significant amounts rutin, gallic acid, caffeic acid, and chlorogenic acid. Despite quantitative differences in the concentration of these bioactive molecules, both extracts increased the viability of cells exposed to H2O2 in concentrations ranging from 300 to 900 μg/mL. Caspases 1, 3, and 8 decreased significantly in cells concomitantly exposed to H2O2 and these extracts, indicating that tucumã cryoprotection involves apoptosis modulation.

KEYWORDS:

Astrocaryum aculeatum; Carotenoids; DNA damage; Lymphocyte culture

PMID:
25466084
DOI:
10.1016/j.foodchem.2014.10.067
[Indexed for MEDLINE]
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