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Food Chem. 2015 Apr 15;173:521-6. doi: 10.1016/j.foodchem.2014.10.087. Epub 2014 Oct 23.

Fast identification of lipase inhibitors in oolong tea by using lipase functionalised Fe3O4 magnetic nanoparticles coupled with UPLC-MS/MS.

Author information

1
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 100049, China.
2
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
3
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; Department of Chemistry and Biochemistry, Jackson State University, 1400 Lynch St., Jackson, MS 39217, USA.
4
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China. Electronic address: liaoxun@cib.ac.cn.

Abstract

Oolong tea is an important member in tea family, which claims for various health benefits such as preventing obesity and improving lipid metabolism. In this work, using pancreatic lipase (PL) functionalised magnetic nanoparticles (PL-MNPs) as solid phase extraction absorbent in combination with ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS), we developed a method for rapid screening and identification of lipase inhibitors from oolong tea. Three PL ligands were selectively extracted and identified as (-)-epigallocatechin-3-O-gallate (EGCG), (-)-gallocatechin-3-O-gallate (GCG) and (-)-epicatechin-3-O-gallate (ECG). Their lipase inhibitory activities were significantly higher than those non-ligands. Structure-activity analysis revealed that the presence of a galloyl moiety in the structure was required for binding to PL-MNPs, and therefore, exhibiting a strong inhibition on the enzyme. Taking advantages of the specificity in enzyme binding and the convenience of magnetic separation, this method has great potential for fast screening of lipase inhibitors from natural resources.

KEYWORDS:

(−)-Epicatechin-3-O-gallate (PubChem CID: 107905); (−)-Epigallocatechin-3-O-gallate (PubChem CID: 65064); (−)-Gallocatechin-3-O-gallate (PubChem CID: 44257114); Ligand fishing; Lipase inhibitors; Magnetic nanoparticles; Oolong tea; Pancreas lipase; UPLC–MS

PMID:
25466054
DOI:
10.1016/j.foodchem.2014.10.087
[Indexed for MEDLINE]

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