Format

Send to

Choose Destination
Biomaterials. 2015 Jan;39:155-63. doi: 10.1016/j.biomaterials.2014.10.076. Epub 2014 Nov 21.

USPIO-labeled textile materials for non-invasive MR imaging of tissue-engineered vascular grafts.

Author information

1
Dept. of Experimental Molecular Imaging, University Clinic, RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany.
2
Dept. of Tissue Engineering & Textile Implants, Applied Medical Engineering, Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Pauwelsstrasse 20, 52074 Aachen, Germany.
3
Institut für Textiltechnik, RWTH Aachen University, Otto-Blumenthal-Strasse 1, 52074 Aachen, Germany.
4
Dept. of Experimental Molecular Imaging, University Clinic, RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany; Institute for Molecular Cardiovascular Research, University Clinic, RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany.
5
Institute for Molecular Cardiovascular Research, University Clinic, RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany.
6
Dept. of Tissue Engineering & Textile Implants, Applied Medical Engineering, Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Pauwelsstrasse 20, 52074 Aachen, Germany; Institut für Textiltechnik, RWTH Aachen University, Otto-Blumenthal-Strasse 1, 52074 Aachen, Germany. Electronic address: Stefan.Jockenhoevel@ita.rwth-aachen.de.
7
Dept. of Experimental Molecular Imaging, University Clinic, RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany. Electronic address: fkiessling@ukaachen.de.
8
Dept. of Experimental Molecular Imaging, University Clinic, RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany; Dept. of Controlled Drug Delivery, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands. Electronic address: tlammers@ukaachen.de.

Abstract

Non-invasive imaging might assist in the clinical translation of tissue-engineered vascular grafts (TEVG). It can e.g. be used to facilitate the implantation of TEVG, to longitudinally monitor their localization and function, and to provide non-invasive and quantitative feedback on their remodeling and resorption. We here incorporated ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles into polyvinylidene fluoride (PVDF)-based textile fibers, and used them to prepare imageable tissue-engineered vascular grafts (iTEVG). The USPIO-labeled scaffold materials were molded with a mixture of fibrin, fibroblasts and smooth muscle cells, and then endothelialized in a bioreactor under physiological flow conditions. The resulting grafts could be sensitively detected using T1-, T2- and T2*-weighted MRI, both during bioreactor cultivation and upon surgical implantation into sheep, in which they were used as an arteriovenous shunt between the carotid artery and the jugular vein. In vivo, the iTEVG were shown to be biocompatible and functional. Post-mortem ex vivo analyses provided evidence for efficient endothelialization and for endogenous neo-vascularization within the biohybrid vessel wall. These findings show that labeling polymer-based textile materials with MR contrast agents is straightforward and safe, and they indicate that such theranostic tissue engineering approaches might be highly useful for improving the production, performance, personalization and translation of biohybrid vascular grafts.

KEYWORDS:

MRI; Textile material; Tissue engineering; USPIO; Vascular graft

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center