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Nutr Res. 2015 Feb;35(2):127-35. doi: 10.1016/j.nutres.2014.10.011. Epub 2014 Oct 30.

Vegetable peptones increase production of type I collagen in human fibroblasts by inducing the RSK-CCAAT/enhancer binding protein-β phosphorylation pathway.

Author information

1
Biospectrum Life Science Institute, Seongnam City, 462-807 Gyeonggi-Do, Republic of Korea.
2
Department of Genetic Engineering, Sungkyunkwan University, Suwon-Si, 440-746 Gyeonggi-Do, Republic of Korea.
3
Department of Physical Therapy, College of Health Science, Eulji University, Seongnam City, 461-713 Gyeonggi-Do, Republic of Korea.
4
Department of Senior Healthcare, BK21 plus program, Graduated school, Eulji University, Seongnam City, 461-713 Gyeonggi-Do, Republic of Korea.
5
Department of Life Science, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, Seongnam-Si, 461-701 Gyeonggi-Do, Republic of Korea. Electronic address: leesaye@gachon.ac.kr.
6
Department of Dermatological Health Management, College of Health Science, Eulji University, Seongnam City, 461-713 Gyeonggi-Do, Republic of Korea; Department of Senior Healthcare, BK21 plus program, Graduated school, Eulji University, Seongnam City, 461-713 Gyeonggi-Do, Republic of Korea. Electronic address: jslee@eulji.ac.kr.

Abstract

Skin aging appears to be principally attributed to a decrease in type I collagen level and the regeneration ability of dermal fibroblasts. We hypothesized that vegetable peptones promote cell proliferation and production of type I collagen in human dermal fibroblasts. Therefore, we investigated the effects of vegetable peptones on cell proliferation and type I collagen production and their possible mechanisms in human dermal fibroblasts. Vegetable peptones significantly promoted cell proliferation in a concentration-dependent manner. In addition, the human luciferase type I collagen α2 promoter and type I procollagen synthesis assays showed that the vegetable peptones induced type I procollagen production by activating the type I collagen α2 promoter. Moreover, the vegetable peptones activated p90 ribosomal s6 kinase, which was mediated by activating the Raf-p44/42 mitogen-activated protein kinase signaling pathway. Furthermore, the vegetable peptone-induced increase in cell proliferation and type I collagen production decreased upon treatment with the ERK inhibitor PD98059. Taken together, these findings suggest that increased proliferation of human dermal fibroblasts and enhanced production of type I collagen by vegetable peptones occur primarily by inducing the p90 ribosomal s6 kinase-CCAAT/enhancer binding protein β phosphorylation pathway, which is mediated by activating Raf-ERK signaling.

KEYWORDS:

Cell proliferation; Human dermal fibroblasts; RSK pathway; Skin aging; Type I procollagen; Vegetable peptones

PMID:
25464949
DOI:
10.1016/j.nutres.2014.10.011
[Indexed for MEDLINE]

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