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Cell Host Microbe. 2014 Dec 10;16(6):748-58. doi: 10.1016/j.chom.2014.10.018. Epub 2014 Nov 26.

Modulation of RNA polymerase II phosphorylation downstream of pathogen perception orchestrates plant immunity.

Author information

1
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; Department of Plant Pathology & Microbiology, and Institute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX 77843, USA.
2
Department of Biochemistry & Biophysics, and Institute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX 77843, USA.
3
Department of Plant Pathology & Microbiology, and Institute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX 77843, USA; Department of Biochemistry & Biophysics, and Institute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX 77843, USA; Center of Biosciences & Biotechnology, North Rio de Janeiro State University, 28013-602, Brazil.
4
Department of Plant Pathology & Microbiology, and Institute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX 77843, USA.
5
Department of Plant Pathology & Microbiology, and Institute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX 77843, USA; Center of Biosciences & Biotechnology, North Rio de Janeiro State University, 28013-602, Brazil.
6
Department of Biochemistry & Biophysics, and Institute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX 77843, USA; Department of Biology, University of Puerto Rico, Mayagüez Campus, Mayagüez, PR 00680, USA.
7
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China; Department of Biochemistry & Biophysics, and Institute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX 77843, USA.
8
Department of Biology, University of Florida, Gainesville, FL 32610, USA.
9
Institute for Bioscience & Biotechnology Research, University of Maryland, Rockville, MD 20850; Department of Plant Science & Landscape Architecture, University of Maryland, College Park, MD 20742, USA.
10
Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, NY 14853, USA.
11
Genomics and Bioinformatics Services, Texas A&M AgriLife Research, College Station, TX 77845, USA.
12
Vegetable and Fruit Improvement Center, Department of Horticultural Sciences, Texas A&M University, College Station, TX 77843, USA.
13
College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
14
Center of Biosciences & Biotechnology, North Rio de Janeiro State University, 28013-602, Brazil.
15
Department of Plant Pathology & Microbiology, and Institute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX 77843, USA. Electronic address: lshan@tamu.edu.
16
Department of Biochemistry & Biophysics, and Institute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX 77843, USA. Electronic address: pinghe@tamu.edu.

Abstract

Perception of microbe-associated molecular patterns (MAMPs) elicits host transcriptional reprogramming as part of the immune response. Although pathogen perception is well studied, the signaling networks orchestrating immune gene expression remain less clear. In a genetic screen for components involved in the early immune gene transcription reprogramming, we identified Arabidopsis RNA polymerase II C-terminal domain (CTD) phosphatase-like 3 (CPL3) as a negative regulator of immune gene expression. MAMP perception induced rapid and transient cyclin-dependent kinase C (CDKC)-mediated phosphorylation of Arabidopsis CTD. The CDKCs, which are in turn phosphorylated and activated by a canonical MAP kinase (MAPK) cascade, represent a point of signaling convergence downstream of multiple immune receptors. CPL3 directly dephosphorylated CTD to counteract MAPK-mediated CDKC regulation. Thus, modulation of the phosphorylation dynamics of eukaryotic RNA polymerase II transcription machinery by MAPKs, CTD kinases, and phosphatases constitutes an essential mechanism for rapid orchestration of host immune gene expression and defense upon pathogen attacks.

PMID:
25464831
PMCID:
PMC4268009
DOI:
10.1016/j.chom.2014.10.018
[Indexed for MEDLINE]
Free PMC Article

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